Decoding metabolic trade-offs in <i>Halomonas elongata</i>: Chemostat-based flux remodeling for industrial ectoine biosynthesis.

ABSTRACT: Halomonas elongata, a moderately halophilic γ-proteobacterium of industrial interest, serves as a microbial cell factory for ectoine-a high-value compatible solute extensively utilized in biopharmaceuticals and cosmetics. While its ectoine biosynthesis potential is well-documented, the systemic metabolic adaptations underlying osmoadaptation remain poorly characterized, limiting rational engineering strategies for optimized production. To address this gap, we employed chemostat cultivation coupled with multi-omics integration (physiological profiling, metabolomics, and metabolic flux analysis) to dissect salt-dependent metabolic network rewiring in the model strain DSM 2581^T under moderate (6.0 % NaCl) and high salinity (13.0 % NaCl). Results demonstrated that, und

SUBMITTER: Yu J 

PROVIDER: S-EPMC12318276 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature