Photoactivation of BODIPY Fluorescence with Green Light.

ABSTRACT: Existing synthetic dyes with photoactivatable fluorescence demand ultraviolet radiation or, at best, violet light for fluorescence photoactivation. Illumination of biological samples within this range of relatively short wavelengths, however, causes significant photodamage. Strategies for the photochemical generation of fluorescent products under irradiation at wavelengths longer than 500 nm with moderate power densities are urgently needed to enable live-cell imaging with negligible phototoxicity. We identified a possible structural design to satisfy these stringent irradiation requirements. Specifically, we demonstrated that illumination of a borondipyrromethene (BODIPY) chromophore in the green region of the visible spectrum cleaves an adjacent oxazine heterocycle to form a fluorescent

SUBMITTER: Tomassini A 

PROVIDER: S-EPMC12318360 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature