Optimizing carbon nanoparticle staining for sentinel lymph nodes in rabbit lower limb models: concentration and time dependence.

ABSTRACT:

Background and objectives

Carbon nanoparticles (CNPs) have been used as a lymphatic imaging agent that can selectively enter lymphatic vessels. This experiment used CNPs for lower limb lymph node staining in rabbits, aiming to explore the suitable concentration and time of CNPs for sentinel lymph node (SLN) sufficient staining, and the biosafety of CNPs retention was preliminarily explored.

Methods

Staining effect of SLN using different concentrations of CNP injection on the rabbit lower limb lymphatic model. To explore the temporal correlation of CNPs staining. We investigated the effects of long-term retention of CNPs in lymph nodes on lymph node tissue structure and mRNA expression of inflammatory factors (IL-6/TNF-α), apoptosis factors (Bcl-2/Bax), and fibrosis factors (

SUBMITTER: Kylyshbek N 

PROVIDER: S-EPMC12318498 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature