The Anti-Angiogenic Effect of Microbotox on Rosacea Is Due to the Suppressed Secretion of VEGF by Mast Cells Resulting From Internalization of the MRGPRX2 Receptor.

ABSTRACT:

Background

Intradermal microdroplet injections of botulinum toxin type-A (BoNT/A) effectively ameliorate rosacea-related angiogenesis, but the mechanism remains unclear.

Objective

To explore the anti-angiogenesis of BoNT/A in the rosacea-like mouse model and to measure the secretion of vascular endothelial growth factor (VEGF) by mast cells.

Methods

A rosacea-like mouse model was induced by LL37 in both Mas-related G-protein-coupled receptor B2 conditional knockout (MrgprB2^-/-) mice and wild-type (WT) mice, then treated with BoNT/A and/or Apatinib. The abundance of endothelial cells and mast cells in mouse skin was determined using dual immunofluorescence staining. The VEGF levels in supernatants and cell lysates of laboratory of allergic disease 2 (LAD2) m

SUBMITTER: Wan J 

PROVIDER: S-EPMC12318782 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature