Distribution of human-pathogenic <i>Cryptosporidium</i> spp., <i>Giardia duodenalis</i>, and <i>Enterocytozoon bieneusi</i> in crab-eating macaques in China.

ABSTRACT:

Introduction

The positive rates and genetic identity of Cryptosporidium spp., Giardia duodenalis (G. duodenalis), and Enterocytozoon bieneusi (E. bieneusi) were unclear in crab-eating macaques in Suzhou and Beijing, China.

Methods

A total of 504 fecal samples were collected from crab-eating macaques on commercial farms in Beijing and Suzhou, China. The extracted DNA was analyzed for Cryptosporidium spp. and E. bieneusi by nested PCR and sequence analysis of the small subunit rRNA (SSU rRNA) gene and the internal transcribed spacer (ITS) gene, respectively. The G. duodenalis was detected by nested PCR targeting β-giardin (bg) gene, glutamate dehydrogenase (gdh) gene, and triosephosphate isomer

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12318935 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature