Copper enhances tetracycline resistance via the efflux transporter CrdAB-CzcBA in <i>Helicobacter pylori</i>.

ABSTRACT: Helicobacter pylori infection is a significant risk factor for various gastrointestinal diseases, while the standard triple therapy for its eradication is increasingly compromised by antibiotic resistance. This study investigates the role of the CrdAB-CzcBA efflux pump and its regulation by copper in tetracycline resistance in H. pylori. Using minimum inhibitory concentration (MIC) determination and growth curve analysis, we found that the deletion of crdA or czcA significantly reduced tetracycline resistance, while overexpression of CrdAB-CzcBA under the urease promoter enhanced bacterial resistance by reducing intracellular tetracycline accumulation. Ethidium bromide and tetracycline accumulation assays confirmed that CrdAB-CzcBA mediates active efflux of tetr

SUBMITTER: Gao F 

PROVIDER: S-EPMC12319023 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature