The TonB-Dependent Transport System Facilitates the Uptake of Inorganic Metal Mediators in Pseudomonas putida KT2440 in a Bioelectrochemical System.

ABSTRACT: Mediator-based extracellular electron transfer (EET) in a bioelectrochemical system is a unique approach to regulate the microbial redox and energy metabolism of Pseudomonas putida KT2440, which enables a new-to-nature high product yield under anaerobic conditions. Previous studies identified respiratory complex III in the inner membrane as a key redox protein involved in mediator (ferricyanide) interactions, but the exact mechanism through which the mediator crosses the outer membrane to extract electrons from membrane-bound redox proteins and transfer them to the anode remains unclear. In this study, we demonstrated the critical role of the TonB-dependent system, a widespread transportation system in gram-negative bacteria, in the mediator-based EET process. Transcriptomic analyses revea

SUBMITTER: Weimer A 

PROVIDER: S-EPMC12319065 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature