High-fidelity tissue super-resolution imaging achieved with confocal² spinning-disk image scanning microscopy.

ABSTRACT: Super-resolution imaging has revolutionized our ability to visualize biological structures at subcellular scales. However, deep-tissue super-resolution imaging remains constrained by background interference, which leads to limited depth penetration and compromised imaging fidelity. To overcome these challenges, we propose a novel imaging system, confocal² spinning-disk image scanning microscopy (C²SD-ISM). It integrates a spinning-disk (SD) confocal microscope, which physically eliminates out-of-focus signals, forming the first confocal level. A digital micromirror device (DMD) is employed for sparse multifocal illumination, combined with a dynamic pinhole array pixel reassignment (DPA-PR) algorithm for ISM super-resolution reconstruction, forming the second confocal level. The

SUBMITTER: Liang Q 

PROVIDER: S-EPMC12319088 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature