Impact of tumor necrosis factor-alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three-dimensional collagen hydrogel.

ABSTRACT:

Background

Advanced ovarian cancer involves tumor cells proliferating within ascitic fluid, which is rich in chemokines, cytokines, growth factors, and phospholipids essential for tumor progression. Tumor necrosis factor-alpha (TNF-α) and lysophosphatidic acid (LPA) are critical components that modulate ovarian tumor cell behavior in vivo, though most in vitro studies have relied on cell monolayers that do not accurately represent the tumor microenvironment. We thus investigated TNF-α and LPA effects on ovarian tumor cells cultured in collagen scaffolds, which are a model more reflective of in vivo conditions.

Methods

Ovarian cancer cell lines SKOV-3, OVCAR-8, OVCAR-5, and OVCAR-4 were cultured in collagen I hydrogels at various concentrations of TNF-α and LPA for 2-8 days.

SUBMITTER: Tino AB 

PROVIDER: S-EPMC12319141 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature