Optimised Ribosome Profiling Reveals New Insights Into Translational Regulation in Synchronised Chlamydomonas reinhardtii Cultures.

ABSTRACT: Deep sequencing of ribosome footprints, also known as ribosome profiling (Ribo-seq), enables the quantification of mRNA translation and a comprehensive view of the translatome landscape. Here, we report an optimised Ribo-seq protocol and analysis pipeline for the model green alga, Chlamydomonas reinhardtiii (Chlamydomonas). Compared to the previously published data sets, the ribosome-protected fragments generated by our protocol showed improved mapping rates to the main open reading frames, reduced bias mapping to the gene coding regions and high 3-nt footprint periodicity. Using this optimised protocol, we employed Ribo-seq alongside RNA-seq to compute translation efficiency and identify genes with differential translation during the diurnal cycle. Interestingly, we found that the transla

SUBMITTER: Lin YL 

PROVIDER: S-EPMC12319295 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature