Mitochondrial bioenergetics deficiency in cisd-1 mutants is linked to AMPK-mediated lipid metabolism.

ABSTRACT:

Background

CISD-1 is a mitochondrial iron-sulfate [2Fe-2S] protein known to be associated with various human diseases, including cancer and diabetes. Previously, we demonstrated that CISD-1 deficiency in worms lowers glucose and ATP levels. In this study, we further explored how worms compensate for lower ATP levels by analyzing changes in cytoplasmic and mitochondrial iron content, AMPK activities, and total lipid profiles.

Materials and methods

Expression levels of CISD-1 and CISD-1GFP fusion proteins in wild-type worms (N2), cisd-1-deletion mutants (tm4993 and syb923) and GFP insertion transgenic worms (PHX953 and SJL40) were examined by Western blot. Fluorescence microscopy analyzed CISD-1GFP pattern in PHX953 embryos and adults, and lipid droplet sizes in N2, cisd-1, aa

SUBMITTER: Hsiung KC 

PROVIDER: S-EPMC12319338 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature