Role of cellular prion protein in mouse granulosa cells and its effects on ovarian function in knockout mice.

ABSTRACT: Cellular prion protein (PrP^c) regulates ovarian reserve maintenance through anti‑Müllerian hormone (AMH)‑dependent mechanisms. The present study explored the role of PrP^c in the ovarian function of mice using complementary in vitro and in vivo models. First, prion protein gene (PRNP) knockdown or overexpression was carried out in mouse ovarian granulosa cells. In vitro analyses conducted using flow cytometry and ELISA revealed that the depletion of PrP^c specifically hindered the secretion of AMH compared with control groups, while the levels of progesterone (P4) and estradiol (E2) remained unchanged across all experimental groups. Importantly, the reduction in AMH levels was reversed upon re‑expression of PrP^c. Additionally, neither

SUBMITTER: Cao Q 

PROVIDER: S-EPMC12319384 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature