Defining the Ovarian Cancer Precancerous Landscape through Modeling Fallopian Tube Epithelium Reprogramming Driven by Extracellular Vesicles.

ABSTRACT: Serous tubal intraepithelial carcinomas (lesions) in the human fallopian tube epithelium (hFTE) are theorized to give rise to high-grade serous ovarian cancers. Small extracellular vesicles (sEV) are known to mediate key signaling in both normal and cancerous tissues, but few ex vivo systems exist for studying the impact of sEV on hFTE tissue. In this study, we present a microfluidic tissue culture platform with combined spatial transcriptomic and proteomic readouts that allows us to profile dual responses in tissue exposed to sEV "messages"-capturing both short-term transcriptomic shifts in the tissue and long-term changes in protein cargo of secreted EVs (the "reply"). Using spatial transcriptomics, we show that the short-term 1-day exposure to ovarian cancer-derived sEVs alters expressi

SUBMITTER: Sipes J 

PROVIDER: S-EPMC12319521 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature