Optogenetic control of transgene expression in <i>Marchantia polymorpha</i>.

ABSTRACT:

Premise

The model liverwort Marchantia polymorpha is an emerging testbed species for plant metabolic engineering but lacks well-characterized inducible promoters, which are necessary to minimize biochemical and physiological disruption when over-accumulating target products. Here, we demonstrate the functionality of the light-inducible plant-usable light-switch elements (PULSE) optogenetic system in Marchantia and exemplify its use through the light-inducible overproduction of the bioplastic poly-3-hydroxybutyrate (PHB).

Methods

The PULSE system was used to drive expression of luciferase as a reporter and characterize its induction in transgenic M. polymorpha. Additionally, PULSE was used to drive expression of the PHB biosynthetic pathway; the accumulat

SUBMITTER: Lindstrom Battle AL 

PROVIDER: S-EPMC12319710 | biostudies-literature | 2025 Jul-Aug

REPOSITORIES: biostudies-literature