Stabilization of OLFML1 via m⁶A Reader IGF2BP3 Drives CSC Characteristics Through Hedgehog Pathway Activation in CRC.

ABSTRACT: Colorectal cancer (CRC) progression is closely associated with cancer stemness, which contributes to poor prognosis and therapeutic resistance. This study identifies OLFML1 as a key target accounting for CRC progression. High expression of OLFML1 promotes CRC cell proliferation and cancer stemness. As for mechanism study, we further revealed that IGF2BP3 as a critical up-stream regulator of OLFML1. Our study indicated that IGF2BP3 stabilizes OLFML1 mRNA through m⁶A modification, thereby enhancing its expression. In addition, IGF2BP3 prevents OLFML1 degradation via the ubiquitin-proteasome pathway. Clinically, this study demonstrated a positive association between IGF2BP3 and OLFML1 in CRC patient samples. High co-expression of IGF2BP3 and OLFML1 was significantly correlated with

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC12320246 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature