Identification of IQGAP3 prognostic potential and involvement in immune cell infiltration in LUAD.

ABSTRACT:

Background

Although IQGAP3 has been identified as a prognostic biomarker in various cancers, its mechanistic role in lung adenocarcinoma (LUAD) progression and modulation of the tumor immune microenvironment remains poorly defined.

Methods

We integrated bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) analyses of clinical specimens to quantify IQGAP3 expression in LUAD. Survival analyses correlated expression levels with patient outcomes. Functional assays including CCK-8, colony formation, EdU incorporation, migration, and invasion assays were performed to evaluate the role of IQGAP3 in LUAD cells. Enrichment analyses (KEGG, GO, and GSEA) were used to identify relevant biological pathways. Immune infiltration analyses evaluated correlations with cytotoxic T-ce

SUBMITTER: Chen M 

PROVIDER: S-EPMC12320309 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature