A <i>NOTCH4</i> intron 28 regulatory element controls arterial specification and lymphoid development from hPSCs.

ABSTRACT: NOTCH signaling plays a critical role in arterial specification, and the formation of transient definitive lymphomyeloid progenitors and hematopoietic stem cells from hemogenic endothelium (HE). To investigate NOTCH signaling mechanisms critical for arterial and lymphoid specification, we performed single-cell multiomics analysis of human pluripotent stem cells (hPSCs) at different stages of hematopoietic differentiation. These studies uncovered the activation of NOTCH signaling and the arterial program, along with dynamic changes in related regulons throughout the HE specification and the endothelial-to-hematopoietic transition. We revealed that expression of NOTCH4 in the arterial endothelium is controlled by a cis-regulatory element within intron 28 (NOTCH4in28) through SO

SUBMITTER: Jung HS 

PROVIDER: S-EPMC12320403 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature