Oncogenic lncRNA transgene transcription modulates epigenetic memory at a naive chromosomal locus.

ABSTRACT: Maintaining genome integrity is essential for the proper functioning and development of organisms. An intriguing aspect is that neocentromeres can form at non-centromeric sites. CENP-A, a key epigenetic marker of centromeres, is often mislocalized to ectopic sites in cancers when overexpressed. Its deposition on centromeres relies on transcription of centromeric non-coding RNAs. Subsequently, ectopic CENP-A is frequently found at transcriptionally active and chromosome breakpoint regions. We previously engineered a stable ectopic CENP-A site on a naïve chromosome by overexpressing PCAT2, a non-centromeric oncogenic lncRNA that recruits CENP-A to its transcribing locus. We tracked cells with this transgene to analyze the longevity of ectopic CENP-A. We discovered that this induced epigeneti

SUBMITTER: Sikder S 

PROVIDER: S-EPMC12320815 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature