Programmable protein ligation on cell surfaces.

ABSTRACT: The surface landscapes of cells differ as a function of cell type and are frequently altered in disease contexts^1-3. Exploiting such differences is key to many therapeutic strategies and is the basis for developing diagnostic and basic-science tools. State-of-the-art strategies typically target single surface antigens, but each individual receptor rarely defines the specific cell type^4,5. The development of programmable molecular systems that integrate multiple cell-surface features to convert on-target inputs to user-defined outputs is therefore highly desirable. Here we describe an autonomous decision-making device driven by proximity-gated protein trans-splicing that allows local generation of an active protein from two otherwise inactive polypeptide fragments. We

SUBMITTER: Kofoed C 

PROVIDER: S-EPMC12321220 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature