Engineering the TCA cycle regulator GarA to increase erythromycin production in <i>Saccharopolyspora erythraea</i>.

ABSTRACT: Actinobacteria are important for industrial production of antibiotics, fine chemicals and food and a source of new compounds for drug discovery. Their central metabolism is regulated by a conserved protein GarA that is unique to the Actinobacteria and has been studied in Mycobacterium tuberculosis and Corynebacterium glutamicum. GarA regulates the TCA cycle and glutamate metabolism by direct binding to enzymes to modulate their activity on glutamate and alpha-ketoglutarate. Given the importance of the TCA cycle in the synthesis of acyl-CoA precursors for antibiotic biosynthesis, and increasing evidence for the role of nitrogen regulators in control of secondary metabolism, we hypothesized that engineering GarA could be used to enhance production of valuable metabolites. His

SUBMITTER: Liuzzi AD 

PROVIDER: S-EPMC12321487 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature