Initial shoot regeneration in the selenium hyperaccumulator Neptunia amplexicaulis and in vitro test system for selenium tolerance and accumulation.

ABSTRACT: The trace element selenium is essential for human nutrition but is distributed unevenly in soils worldwide with extensive selenium-deficient regions and selenium-enriched (seleniferous) areas. Neptunia amplexicaulis is one of the strongest selenium hyperaccumulator plants known and native to Australian seleniferous soils. Research in the genetic background of the selenium accumulation and tolerance mechanisms of this species lacks biotechnological and molecular tools for functional genetics. Therefore, this study aimed to develop a de novo shoot regeneration protocol for N. amplexicaulis and validate an selenium accumulation test system. Callus was induced on root and hypocotyl explants excised from 5-day old seedlings and cultured on an adjusted MS medium (SIM9) containing 4.5 µM Thidiazu

SUBMITTER: Buhmann B 

PROVIDER: S-EPMC12321714 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature