Relationship between AQP5 and chemotherapy resistance in colorectal cancer cells and its mechanism.

ABSTRACT:

Background

Previous studies have suggested that AQP5 expression is increased in colorectal cancer tissues and is associated with the progression and prognosis of colorectal cancer. However, there are few studies on the relationship between AQP5 and chemotherapy resistance in colorectal cancer cells and the related mechanisms.

Methods

In this study, AQP5 overexpression plasmid was transfected into human colorectal cancer cell lines RKO and HCT116, and the effects of AQP5 combined with 5-FU on the proliferation and apoptosis of colorectal cancer cells and the underlying mechanism were investigated by western blotting, MTT assay and flow cytometry. Then, the results of in vitro experiments were verified in vivo using SPF nude mice.

Results

AQP5 overexpression plasmid tra

SUBMITTER: Yang X 

PROVIDER: S-EPMC12321721 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature