MicroRNA-27b alleviates septic cardiomyopathy by targeting the Mff/MAVS axis.

ABSTRACT:

Objective

To investigate the protective role of microRNA-27b (miR-27b) in septic cardiomyopathy (SCM) and its regulatory mechanism on the mitochondrial fission factor (Mff)/mitochondrial antiviral signaling protein (MAVS) axis.

Methods

Transcriptome data from septic patients' cardiac tissues (GSE79962) were analyzed. Serum miR-27b expression was measured in SCM patients (n=11), sepsis-only patients (n=22), and healthy controls (n=30). Mouse SCM model and HL-1 cardiomyocyte model were established by lipopolysaccharide (LPS) induction. The molecular mechanism was investigated using miR-27b agonist/antagonist and Mff intervention, combined with RT-qPCR, Western blot, immunofluorescence, and transmission electron microscopy.

Results

Bioinformatics analysis revealed signif

SUBMITTER: Wang X 

PROVIDER: S-EPMC12321784 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature