Pathway metabolite ratios reveal distinctive glutamine metabolism in a subset of proliferating cells.

ABSTRACT: Large-scale metabolomic analyses of pan-cancer cell line panels have provided significant insights into the relationships between metabolism and cancer cell biology. Here, we took a pathway-centric approach by transforming targeted metabolomic data into ratios to study associations between reactant and product metabolites in a panel of cancer and non-cancer cell lines. We identified five clusters of cells from various tissue origins. Of these, cells in Cluster 4 had high ratios of TCA cycle metabolites relative to pyruvate, produced more lactate yet consumed less glucose and glutamine, and greater OXPHOS activity compared to Cluster 3 cells with low TCA cycle metabolite ratios. This was due to more glutamine cataplerotic efflux and not glycolysis in cells of Cluster 4. In silico analyses o

SUBMITTER: Santiappillai NT 

PROVIDER: S-EPMC12322234 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature