Techno-Economic Analysis of Membrane-Based Purification Platforms for AAV Vector Production.

ABSTRACT: Technologies for large-scale manufacturing of viral vectors for gene therapies, such as tangential flow filtration and membrane chromatography, are under development. In these early stages of process development, techno-economic analyses are useful for identifying membrane properties yielding the greatest impact on process performance. In this study, we adapted a techno-economic framework used for monoclonal antibody capture for adeno-associated viral vector purification. We added mechanistic models to simulate flux decline during harvesting and separating full and empty capsids during polishing. Graphical user interfaces were added to help users explore the design search space. We selected a base process and manipulated selected variables to see their impact on large-scale manufacturing p

SUBMITTER: Romero JJ 

PROVIDER: S-EPMC12322620 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature