Spatial and Diurnal Variations in Sodium Content Within Intervertebral Disc Tissue.

ABSTRACT:

Background

Daily cycles in tissue osmolarity within the intervertebral disc (IVD) are a biophysical signal that regulates cellular metabolism and arise from deformation of the charged and hydrated extracellular matrix (ECM). However, the magnitude of these osmotic cycles remains unclear due to spatial variations in ECM composition and fixed charge density (FCD) between the regions of the IVD and between the pericellular matrix (PCM) and surrounding ECM. This study aimed to (1) validate the use of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to assess intra-tissue sodium content and (2) apply this method to assess temporal and spatial changes in intra-tissue sodium content during a diurnal loading cycle.

Methods

The FCD of nucleus pulposus (NP) tiss

SUBMITTER: Marshall JT 

PROVIDER: S-EPMC12322741 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature