Proteomic Profiling of Acoustically Isolated Extracellular Vesicles from Blood Plasma during Murine Bacterial Sepsis.

ABSTRACT: Sepsis is a life-threatening condition caused by a dysregulated host response to an infection and is a leading cause of death worldwide. The condition is variable, which in combination with insufficient clinical markers, makes it challenging to predict when infection will progress to sepsis and to categorize patients into homogeneous patient subgroups. In this study, we demonstrate the use of acoustic trapping to rapidly enrich extracellular vesicles (EVs) from minute volumes of blood plasma from experimental mouse models of sepsis infected with the Gram-positive pathogen Streptococcus pyogenes or the Gram-negative pathogen Escherichia coli. Using quantitative mass spectrometry-based proteomics, we characterized the proteome of EVs and plasma to demonstrate that the EVs expan

SUBMITTER: Broman A 

PROVIDER: S-EPMC12322956 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature