Comparative performance of Kato-Katz, POC-CCA and real-time PCR in detecting Schistosoma mansoni infection at different endemicity settings in northwest Ethiopia: a cross-sectional study.

ABSTRACT:

Background

Effective detection of Schistosoma mansoni is crucial for the control and management of the infection. This study aimed to evaluate the field performance of three diagnostic methods-point-of-care circulating cathodic antigen (POC-CCA) assay, Kato-Katz (KK) technique, and real-time polymerase chain reaction (RT-PCR)-for detecting S. mansoni infection in different transmission settings across northwest Ethiopia.

Methods

From February to June 2023, a cross-sectional study was conducted in the Amhara Regional State of Ethiopia, involving 1192 randomly selected participants. Stool samples were analyzed using the KK technique and RT-PCR, while urine samples were tested using the POC-CCA cassette. The performance of POC-CCA and RT-PCR was evaluated against a KK reference

SUBMITTER: Alemu G 

PROVIDER: S-EPMC12323140 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature