Adipose tissue-associated Krt23+fibroblasts contribute to immune microenvironment disorders in mouse adipose tissue during the development of obesity.

ABSTRACT: Obesity alters the adipose tissue (AT) immune microenvironment (TIME) and induces inflammation. Nevertheless, the specific time when TIME dysfunction emerges in AT remains elusive. Obesity mouse models were constructed and categorized into Lean (Ctrl), moderately obese (Mid_Ob, with body weight exceeding Ctrl by > 10%), and Obese (Ob, with body weight exceeding Ctrl by > 20%) groups. Single-nucleus RNA sequencing (snRNA-seq) and immunofluorescence localization were employed to analyze changes in cell populations, cluster marker genes, and signaling pathways in mouse epididymal white adipose tissue (eWAT). A total of 23,068 cells obtained from three mouse eWAT samples (Ctrl, Mid_Ob, and Ob) underwent snRNA-seq. Eighteen distinct clusters were identified, and seven cell types were annotated

SUBMITTER: Lu Z 

PROVIDER: S-EPMC12323179 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature