Oral delivery of GLP-1 peptide using recombinant <i>Lactobacillus gasseri</i> for the treatment of type 2 diabetes mellitus.

ABSTRACT: Glucagon-like peptide-1 (GLP-1) is an effective therapeutic peptide for the treatment of type 2 diabetes mellitus (T2DM). Here, we constructed an effective probiotic-based oral GLP-1 delivery system by engineering the probiotic strain of Lactobacillus gasseri (Lgs^GPA) to secrete GLP-1 fusion peptide, which combines GLP-1 with protein transduction domain (PTD) and a serum albumin binding peptide (ABP), GLP-1-PTD-ABP (GPA). The supernatants of Lgs^GPA cultures significantly upregulated the expression of PDX-1 and stimulated insulin release in Min6 cells. Daily oral administration of Lgs^GPA in db/db mice significantly alleviated insulin resistance, hyperglycemia, and dyslipidemia. Simultaneously, the abundance of unclassifi

SUBMITTER: Ke Z 

PROVIDER: S-EPMC12323307 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature