Multisite binding of bacteriophages on lipopolysaccharides in <i>Escherichia coli</i> O157:H7 and the adaptive costs of phage resistance.

ABSTRACT: Escherichia coli (E. coli) O157:H7 is a major foodborne pathogen and a critical global food safety concern. Bacteriophage (phage) therapy offers a promising, highly specific biological alternative to antibiotic treatment. However, the clinical application of phage therapy is frequently limited by the rapid emergence of bacterial resistance. In this study, we examined the interaction between E. coli O157:H7 strain EDL933 and two T4-like phages, assessing the adaptive costs incurred by host strains in developing phage resistance. Our results indicate that phage PSD2001 utilizes capsular polysaccharide and lipopolysaccharides as adsorption receptors, while phage PNJ212 targets outer membrane protein C (OmpC) as its adsorption receptor. Notably, both phage employ gp37-like

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12323348 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature