Immunological and inflammatory responses in the kidneys in experimental acanthamoebiasis.

ABSTRACT: The pathomechanisms of Acanthamoeba spp. infection are still poorly understood. The aim of the study was to determine the expression of the NLR family pyrin domain-containing protein 3 (NLRP3), prostaglandin-endoperoxide synthase 2 (PTGS2, commonly known as cyclooxygenase-2, COX-2), and various cytokines in the kidneys of immunocompetent and immunosuppressed mice infected with Acanthamoeba sp. (T16 genotype). The proteins were analyzed by quantitative reverse transcription PCR. In immunocompetent mice, we observed increased mRNA levels of interferon gamma (IFNγ), tumor necrosis factor alpha (TNFα), PTGS2/COX-2, and interleukin (IL)-17A at the beginning of infection. While in the late stages, we found decreased levels of NLRP3, IL-1β, IL-10, IL-17A, IL-21, IL-23, IFNγ, TNFα, a

SUBMITTER: Kot K 

PROVIDER: S-EPMC12323576 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature