Combining In Vivo 2-Photon Imaging with Photoactivatable Fluorescent Labeling Shows Low Rates of Mitochondrial Dynamics in Skeletal Muscle.

ABSTRACT:

Introduction

Mitochondrial dynamics involve two distinct and opposing processes, fusion and fission. Traditionally, we assess fusion and fission by snapshots of protein markers at distinct time points or in vitro models to infer outcomes in vivo . Recent technological advancements enable visualization of mitochondrial dynamics in vivo using fluorescent microscopy.

Methods

Our study modified this technique to evaluate mitochondrial dynamics in skeletal muscle, comparing young (6mo) and old (24mo) mice in vivo and contrasting this to ex vivo and in vitro models. We hypothesized that in vitro and ex vivo models would have higher rates of dynamics than in vivo models and that young animals would have higher rates than old animals. We electroporated mitochondrial matrix-targeted

SUBMITTER: O'Reilly CL 

PROVIDER: S-EPMC12323594 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature