Biases and complementarity in gut viromes obtained from bulk and virus-like particle-enriched metagenomic sequencing.

ABSTRACT: Due to varying sequencing strategies, current gut virome findings show significant variability. Specifically, bulk- and virus-like particle (VLP)-enriched metagenomic sequencing (termed bulk and VLP, respectively) present unique advantages and limitations, affecting viral genome discovery, taxonomic annotation, and community structure analysis. A comprehensive comparison of these strategies is crucial for thoroughly understanding the gut virome. This study comprehensively compared gut viromes identified from paired bulk and VLP data from 151 adult and 141 infant fecal samples. The VLP method showed superior performance to bulk in viral genome discovery in both data sets by recovering longer and more complete viral genomes, with higher sensitivity for low-abundant ones, resulting in a highe

SUBMITTER: Li Y 

PROVIDER: S-EPMC12323601 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature