Identification of <i>Mycobacterium tuberculosis</i> intracellular survival-related virulence factors via CRISPR-based eukaryotic-like secretory protein mutant library screen.

ABSTRACT: Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), remains a serious infectious disease posing significant global health challenges. A critical evolutionary feature of M.tb is its genome encoding a set of eukaryotic-like secretory proteins, which facilitate intracellular survival by manipulating host immune responses. However, the specific eukaryotic-like secretory proteins that facilitate M.tb intracellular survival and their regulatory mechanisms on host immunity remain uncharacterized. In this study, a mutant library comprising 137 potential eukaryotic-like secretory proteins was constructed using clustered regularly interspaced short palindromic repeats (CRISPR)-non-homologous end joining genome editing technology. Subsequently, macrophages were

SUBMITTER: Liu W 

PROVIDER: S-EPMC12323669 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature