Superparamagnetic Iron Oxide Nanoparticle-Labeled Extracellular Vesicles for Magnetic Resonance Imaging of Ischemic Stroke.

ABSTRACT: Stroke is a leading cause of death and disability worldwide. Extracellular vesicles (EVs) derived from human mesenchymal stem cells (hMSCs) offer a unique and promising alternative to direct cell injection as part of a cell-based therapy for stroke treatment. The development of labeling strategies is essential to identifying the initial biodistribution and clearance of EV-based therapeutics. In this study, hMSC-EVs were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles for magnetic resonance imaging (MRI). Two methods of preparation were evaluated after EVs were sonicated in the presence of USPIO nanoparticle. The labeled EVs were purified by (1) ultracentrifugation only or (2) an extension of a harvesting approach that employs polyethylene glycol (PEG) to enrich E

SUBMITTER: Helsper S 

PROVIDER: S-EPMC12323775 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature