Integrating 12 Spatial and Single Cell Technologies to Characterise Tumour Neighbourhoods and Cellular Interactions in three Skin Cancer Types.

ABSTRACT: Cutaneous squamous cell carcinoma (cSCC), basal cell carcinoma (BCC), and melanoma, the three major types of skin cancer, account for over 70% of all cancer cases. Despite their prevalence, the skin cancer microenvironment remains poorly characterized, both in the outer skin layer where the cancer originates and at the deeper junctional and dermal layers into which it progresses. To address this, we integrated 12 complementary spatial single-cell technologies to construct orthogonally-validated cell signatures, spatial maps, and interactomes for cSCC, BCC, and melanoma. We comprehensively compared and integrated these spatial methods and provided practical guidelines on experimental design. Integrating four spatial transcriptomics platforms, we found keratinocyte cancer signatures, includi

SUBMITTER: Prakrithi P 

PROVIDER: S-EPMC12324176 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature