Subunit-specific roles of LRRC8 proteins in determining glutamate permeability of astrocytic volume-regulated anion channels.

ABSTRACT: Volume-regulated anion channels (VRACs) are ubiquitous chloride channels that play a crucial role in cell volume regulation but are also involved in many other physiological processes. VRACs are heteromers of proteins from the leucine-rich repeat-containing family 8 (LRRC8A-E), with LRRC8A being essential. Other LRRC8 subunits are expressed in a cell type-specific manner and modulate the biophysical properties of VRACs, including permeability to small signaling molecules. Here, we used primary astrocyte cultures from wild-type and genetically modified C57BL/6 mice to investigate (i) LRRC8 subunit composition of endogenous VRACs in the brain and (ii) the subunit determinants of VRAC permeability to the excitatory neurotransmitter glutamate. qPCR and RNA-seq revealed high expression of Lr

SUBMITTER: Chandler ML 

PROVIDER: S-EPMC12324197 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature