Modeling simian immunodeficiency virus (SIV) latency in primary rhesus macaque CD4⁺ T cells.

ABSTRACT: Simian immunodeficiency virus (SIV)-infected rhesus macaques are valuable models for HIV cure research, offering insights into tissue reservoirs and testing reservoir-reduction strategies. Despite this utility, low frequencies of latently infected cells in vivo limit mechanistic studies of viral latency ex vivo. In vitro latency models have addressed this limitation for HIV, advancing our understanding of viral persistence. However, no comparable models exist for SIV. To address this gap, we developed an in vitro model of SIV latency in primary rhesus macaque CD4⁺ T cells, optimizing conditions to promote viral entry while maintaining cells in a minimally activated, non-proliferating state. After 12 days in culture, ~1-3.3% of cells harbored SIV DNA, primari

SUBMITTER: Matschke LM 

PROVIDER: S-EPMC12324229 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature