Bacterial type II topoisomerases cleave DNA in a species-specific manner.

ABSTRACT: The type II topoisomerases, gyrase and topoisomerase IV, are essential enzymes in nearly all bacteria and are the targets of fluoroquinolones, which are some of the most widely prescribed broad-spectrum antibacterials in clinical use. As part of their catalytic cycle, gyrase and topoisomerase IV transiently cleave DNA in a sequence-dependent manner. However, it is unclear whether this sequence-dependence is species-specific. Therefore, using our recently developed SHAN-seq method, we mapped and compared cleavage sites for type II topoisomerases from three different pathogenic bacterial species, Escherichia coli, Bacillus anthracis, and Mycobacterium tuberculosis in the presence of the fluoroquinolone, ciprofloxacin. We found that the enzymes have substantially differen

SUBMITTER: Morgan IL 

PROVIDER: S-EPMC12324340 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature