Histone Acetylation Differentially Modulates CTCF-CTCF Loops and Intra-TAD Interactions.

ABSTRACT: The cohesin complex structures the interphase genome by extruding loops and organizing topologically associating domains (TADs). While cohesin engages chromatin in context-dependent modes, the regulatory influence of chromatin state on these interactions remains unclear. Here, we show that histone hyperacetylation, induced by the histone deacetylase inhibitor trichostatin A (TSA), preferentially disrupts short-range interactions within TADs but spares CTCF-anchored loops, despite reduced cohesin occupancy at these sites. These findings point to two functionally distinct cohesin populations: a TSA-sensitive pool within TADs, likely representing extruding, non-topologically bound cohesin, and a TSA-resistant population at CTCF-CTCF anchors that maintains loops through topological entrapment.

SUBMITTER: Smith RG 

PROVIDER: S-EPMC12324361 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature