Fast photostimulus optimization for holographic control of neural ensemble activity <i>in vivo</i>.

ABSTRACT: Determining the intricate structure and function of neural circuits requires the ability to precisely manipulate circuit activity. Two-photon holographic optogenetics has emerged as a powerful tool for achieving this via flexible excitation of user-defined neural ensembles. However, the precision of two-photon optogenetics has been constrained by off-target stimulation, an effect where proximal non-target neurons can be unintentionally activated due to imperfect spatial confinement of light onto target neurons. Here, we introduce a real-time computational approach to mitigating off-target stimulation by first empirically sampling each neuron's sensitivity to stimulation at proximal locations, and then optimizing stimulation sites using a fast, interpretable model based on adaptive non-nega

SUBMITTER: Triplett MA 

PROVIDER: S-EPMC12324435 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature