Counting cytoplasmic incompatibility factor mRNA using digital droplet PCR.

ABSTRACT: Wolbachia bacteria inhabit over half of all insect species and often spread through host populations via efficient maternal transmission and cytoplasmic incompatibility (CI), killing aposymbiotic embryos when fertilized by symbiotic males. Wolbachia's cifB gene triggers CI in males, while cifA, expressed in females, rescues embryos from CI-induced lethality. In some systems, cifA also contributes to CI induction. CI strength-the percentage of embryos that die from CI-is a key determinant of Wolbachia's prevalence in host populations, and cifB mRNA levels in testes generally correlate with CI strength. Yet, cifB's rarity can hamper precise quantification, necessitating tissue pooling for reverse transcription quantitative PCR (RT-qPCR)

SUBMITTER: Vlaenderen LV 

PROVIDER: S-EPMC12324539 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature