Evaluating Integrative Strategies for Incorporating Phenotypic Features in Spatial Transcriptomics.

ABSTRACT: The key advantage of spatial transcriptomics (ST) technologies lies in the spatial domain: these techniques not only offer an unprecedented opportunity to interrogate intact biological samples in a spatially informed manner, but also set the stage for integration with other imaging-based modalities. However, how to most effectively exploit spatial context and integrate ST with imaging-based modalities that capture morphological insight remains an open and heavily investigated question. To address this, particularly under real-world experimental constraints such as limited dataset size, class imbalance, and bounding-box-based segmentation, we used a publicly available murine ileum Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH) dataset to evaluate whether a minimally t

SUBMITTER: Moser LM 

PROVIDER: S-EPMC12324568 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature