CCDC66 regulation of cytoskeleton and cilia stability is important for signaling and epithelial organization.

ABSTRACT: The primary cilium is a conserved, microtubule-based organelle that transduces signaling pathways essential for development and homeostasis. It dynamically assembles and disassembles in response to intrinsic and extrinsic stimuli while maintaining remarkable structural stability and tightly regulated length. The mechanisms underlying this stability and length control are not well understood. Here, we characterized CCDC66, a microtubule-associated protein linked to ciliopathies, as an important regulator of cilium maintenance and disassembly in mouse epithelial cells. Live imaging revealed that cilia in CCDC66-depleted cells frequently fluctuate in length and exhibit increased cilium disassembly and ectocytosis. Phenotypic rescue experiments and in vitro assays showed that microtubule stabi

SUBMITTER: Deretic J 

PROVIDER: S-EPMC12324684 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature