Bufalin Suppresses Triple-Negative Breast Cancer Stem Cell Growth by Inhibiting the Wnt/β-Catenin Signaling Pathway.

ABSTRACT: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with high mortality rates and limited targeted therapies. TNBC stem cells (TNBCSCs) contribute to tumor aggressiveness, metastasis, and treatment resistance. Targeting TNBCSCs represents a promising therapeutic strategy for improving patient outcomes. In this study, we investigated the inhibitory effects of bufadienolides-bufalin, bufotalin, and cinobufotalin-on TNBCSC growth. Among them, bufalin exhibited the strongest antiproliferative activity. We further examined bufalin's impact on TNBCSC self-renewal, cell cycle regulation, apoptosis, and the Wnt/β-catenin signaling pathway using in vitro and in vivo models. Bufalin effectively suppressed TNBCSC self-renewal in in vitro tumorsphere ass

SUBMITTER: Park SJ 

PROVIDER: S-EPMC12324995 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature