Elucidation of the Hemostatic and Anti-Inflammatory Effects of Walnut Shell Ethanol Extract by Network Pharmacology and Experimental Verification.

ABSTRACT: Effective hemostatic and anti-inflammatory measures are essential for wound healing. In this study, Ultra-performance liquid chromatography (UPLC) combined with network pharmacology was used to analyze the composition of walnut shell ethanol extract (WSEE), and in vivo and in vitro experiments elucidated its potential mechanisms of hemostatic and anti-inflammatory effects. The results showed that 45 components were identified from WSEE, and the NF-κB signaling pathway, IL-17 signaling pathway, etc., could play significant roles in this context. In vitro, WSEE exhibited procoagulant activity including shortened serum APTT (activated partial thromboplastin time, 40.4 ± 3.05 s), TT (thrombin time, 26.13 ± 1.6 s), and PT (prothrombin time, 14.6 ± 2.1 s) (p < 0.01), and increasing FIB le

SUBMITTER: He Y 

PROVIDER: S-EPMC12325102 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature