More than presence-absence; modelling (e)DNA concentration across time and space from qPCR survey data.

ABSTRACT: Environmental DNA (eDNA) surveys offer a revolutionary approach to species monitoring by detecting DNA traces left by organisms in environmental samples, such as water and soil. These surveys provide a cost-effective, non-invasive, and highly sensitive alternative to traditional methods that rely on direct observation of species, especially for protected or invasive species. Quantitative PCR (qPCR) is a technique used to amplify and quantify a targeted DNA molecule, making it a popular tool for monitoring focal species. Modelling of qPCR data has so far focused on inferring species presence/absence at surveyed sites. However, qPCR output is also informative regarding DNA concentration of the species in the sample, and hence, with the appropriate modelling approach, in the environment. In t

SUBMITTER: Jones M 

PROVIDER: S-EPMC12325409 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature