Variability of Bile Baseline Excitation-emission Fluorescence of Two Tropical Freshwater Fish Species.

ABSTRACT: The quantification of pollutant metabolites in fish bile is an efficient approach to xenobiotic pollution monitoring in freshwaters since these measurements directly address exposure. Fluorescence excitation-emission matrix spectroscopy (EEMS) has demonstrated to be a highly specific and cost-effective technique for polycyclic aromatic hydrocarbon (PAH) and PAH-metabolite identification and quantification. EEMS ability to quantify these compounds strongly depends on the intensity and variability of the bile baseline fluorescence (BBF). We found large differences in BBF among Aequidens metae (AME) individuals and of these with Piaractus orinoquensis (PIO). Moreover, BBF was large enough that solvent dilutions of over 1:400 were needed to avoid inner filter effects. We used parallel factor a

SUBMITTER: Mora-Solarte D 

PROVIDER: S-EPMC12325423 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature