Comprehensive lipid structure annotation via photochemical epoxidation and mass spectrometry.

ABSTRACT: Cells tightly regulate lipid structures to fulfill cellular functions and to respond to external stimuli. The biochemical details of the processes that determine lipid structure alterations are often not fully understood. In this manuscript, we present a new epoxidation strategy of unsaturated lipids, which allows the annotation of lipid head group, fatty acid composition, C = C bond position, C = C bond geometry, and sn-isomerism when derivatized lipids are analyzed with corresponding separation and mass spectrometry methods. Tandem mass spectra of epoxidized deprotonated or protonated lipids provide information on head groups, fatty acids, and C = C positions, while MSⁿ of alkali metal adducts reveals sn-isomer compositions. Separation of epoxidation products via reversed-phase liquid ch

SUBMITTER: Yu J 

PROVIDER: S-EPMC12325535 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature